Fig. 5.

Fibroblast priming is accompanied by changes in signalling responses to TNF. BJ fibroblasts were treated for varying times with TNFα (10 ng/mL) with or without a prior 24 h exposure to TNFα and 24 h rest. Cell lysates were prepared and phosphorylated signalling proteins were detected by western blotting. Results are representative of five independent experiments. Vertical lines separate sections from the same gel and exposure. ERK extracellular signal-related kinase, JNK c-Jun N-terminal kinase