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The cytoprotective effect of B. breve is MyD88 dependent. (a,b) IEC MyD88+/+ mice and (c,d) IEC MyD88−/− mice were gavaged with PBS (control) or B. breve UCC2003 and challenged with LPS. Paraffin-embedded intestinal sections were stained with anti-CC3 and quantified using the WinCrypts and Score programs. (e) Columns shown TLR2 expression via RT-PCR. Data are mean ± s.d., n = 12 (two independent experiments) analysed with Mann–Whitney U-test.
