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. 2017 Jan 11;7(1):160212. doi: 10.1098/rsob.160212

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Glycosylation analysis of AtaC_1866–2428 alongside NGT and α6GlcT by anti-dextran and anti-HIS western blots. Top panel: anti-dextran dot blot; (a) dextran; (b) AtaC with NGT K441A and functional α6GlcT; (c) AtaC with functional NGT and α6GlcT; (d) AtaC with functional NGT only. Bottom panel: anti-HIS and anti-dextran Western blots (e,f, respectively); lane 1, AtaC with functional NGT and α6GlcT; lane 2, AtaC with NGT K441A and functional α6GlcT; lane 3, AtaC with functional NGT only.