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(a) NP-HPLC analysis of 2-AB labelled glycans released from purified AtaC which was co-expressed with NGT and α6GlcT in E. coli DH10β (black). A glucose homopolymer (dextran) ladder serves as a reference for retention time (red). The peak originating from excess 2-AB label (rt = 4.1 min) is marked with an asterisk. (b,c) MALDI mass spectrometry analysis of the same glycan sample confirms the identity of the observed glycan chains as a hexose polymer.
