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. 2017 Jan 25;7(1):160258. doi: 10.1098/rsob.160258

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — TGFβ/Activin signalling is required for tissue growth and nucleolar dynamics. (a–c) Salivary glands show a substantial reduction in overall size upon put depletion. Low magnifications images of salivary glands from third-instar Drosophila larvae expressing (a) UAS-lacZ (control), (b) UAS-putRNAi^7904R-3 and (c) UAS-putRNAi³⁷²⁷⁹ under the control of the ey-Gal4 driver. Salivary glands of the indicated genotypes were stained for the cell limits with RhPh (red) and counterstained with DAPI (blue). (d–f) Put requirement for salivary glands growth is linked to an increase in nucleolar size. (d) Localization of the nucleolar marker Fibrillarin in control nuclei of salivary glands. (e) put RNAi^7904R-3 results in ectopic accumulation of Fibrillarin. (f) Strong putRNAi³⁷²⁷⁹ induction results in expansion of the Fibrillarin at the nucleolus. (g) Scatter plot representative of put requirement for salivary gland growth. (h) Salivary gland growth deficit is linked to a decrease in the cellular area. (i) put depletion causes an increase in nucleolar/nuclear area ratio in the salivary glands. (j) Scatter plot showing nuclear area quantification of the described genotypes (n = 25–40; ***p < 1 × 10^–4). Scale bars: (a–c) 200 µm, (d–f) 20 µm.