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. 2017 Jan 25;7(1):160258. doi: 10.1098/rsob.160258

Figure 2.

Figure 2.

Activin R-Smad smad2 is required for nucleolar dynamics. (a–c) TGFβ R-Smads contribution for nucleolar activity. Fibrillarin expansion caused by (a) Activin R-Smad smad2 depletion, (b) BMP R-Smad mad depletion and (c) co-depletion of both R-Smads presents a similar phenotype to smad2RNAi. In all panels, DNA was labelled with DAPI and represented in blue. Scale bars 20 µm. (d–g) Scatter plots showing the quantification of the different organ and cellular parameters after R-Smads depletion. smad2RNAi has a stronger effect on growth levels than madRNAi, both in overall tissue size (d) and cellular area (e). Reduction of the Activin pathway activity increases the relative nucleolar area (f) with a mild impact on the nuclear size (g). (n = 25–40; n.s. means no statistical difference between samples; *p < 0.05; **p < 0.01 and ***p < 1 × 10–4).