Figure 2.

Reduced accuracy mutants accelerate loss of viability during ageing. Data were generated with an RPS2 shuffling strain derived from W303 [15], containing a chromosomal deletion of the RPS2 gene and plasmid-borne copies of either wild-type (black data) or mutant (grey data) copies of the RPS2 gene. (a) Location of the SUP38-5 and SUP38-8 mutations in comparison with other known accuracy mutations in the yeast small ribosomal subunit. (b) Growth rates of RPS2 mutant and wild-type strains in SC−Ura medium. Curves shown represent typical replicates from four independently grown transformants. (c) The SUP38 mutations strongly increase decoding errors as measured with the dual luciferase reporter system. Bars indicate average and standard deviation for five separate transformants. (d) Cells containing RPS2 mutant genes lose viability faster than cells containing the wild-type gene. Shown are averaged data obtained with three separate transformants for each condition.