Figure 4.

Translational accuracy does not change significantly during ageing. Yeast cells were separated by centrifugal counterflow elutriation. (a) Staining of bud scars using FITC-wheat germ agglutinin and determination of average size demonstrates successful separation into fractions of distinct age classes. (b) Dual luciferase reporter measurements reveal no significant changes in stop-codon read-through or in misincorporation of histidine on a CGC (arginine) codon. Bars indicate average and standard deviation of three technical replicates of one elutriation experiment.