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. 2017 Jan 18;7(1):160291. doi: 10.1098/rsob.160291

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — The interaction between translational activity and translational accuracy. Deletion of the TEF1 gene, one of two identical genes encoding elongation factor 1A in yeast, reduces lysine misincorporation on an AGG codon. By contrast, deletion of either of the two identical genes encoding elongation factor 2 increase the same error. Application of low concentrations of cycloheximide, which also blocks the translocation step of translation elongation, has a similar effect as eEF2 depletion. Bars indicate averages and standard deviations for six separate transformants. Statistical significance as determined by one-way ANOVA and post hoc testing (Tukey's HSD) is indicated as follows: n.s., p > 0.05; **p < 0.01; ***p < 0.001.