Table 1.
Peptide sequences used for ricin and RCA quantification and detection. Peptide sequences are listed with corresponding isotopically heavy 13C,15N-labeled peptide standards with the labeled residues denoted with an (*). The monoisotopic precursor m/z are listed with the product ion transitions. Product ion transitions in bold were used for quantification while the others are used for confirmation.
| Quantification | Sequence | Precursor ion | Transition ions monitored | |||
|---|---|---|---|---|---|---|
| TA7, Ricin | VGLPINQR | 448.77 (+2) | 417.22 (+1) y3 | 530.35 (+1) y4 | 627.45 (+1) y5 | |
| VGLPINQ*R | 452.27 (+2) | 424.22 (+1) y3 | 537.35 (+1) y4 | 634.31 (+1) y5 | ||
| TA5, Ricin | LTTGADVR | 416.73 (+2) | 274.19 (+1) y2 | 517.27 (+1) y5 | 618.32 (+1) y6 | |
| LTTGADV*R | 419.73 (+2) | 280.19 (+1) y2 | 523.27 (+1) y5 | 624.32 (+1) y6 | ||
| TB19, Ricin | ASDPSLK | 717.38 (+1) | 260.20 (+1) y2 | 347.23 (+1) y3 | 444.28 (+1) y4 | |
| ASDPSL*K | 724.38 (+1) | 267.20 (+1) y2 | 354.23 (+1) y3 | 451.28 (+1) y4 | ||
| TA6, Ricin, RCA | HEIPVLPNR | 537.81 (+2) | 267.07 (+1) b2 | 695.25 (+1) y6 | 808.47 (+1) y7 | |
| HEIPVLP*NR | 540.81 (+2) | 267.09 (+1) b2 | 701.37 (+1) y6 | 814.49 (+1) y7 | ||
| TA5, RCA | SHLTTGGDVR | 521.77 (+2) | 225.10 (+1) b2 | 705.35 (+1) y7 | 818.44 (+1) y8 | |
| SHLTTGGDV*R | 524.77 (+2) | 225.10 (+1) b2 | 711.35 (+1) y7 | 824.44 (+1) y8 | ||
| TA7, RCA | VGLPISQR | 435.26 (+2) | 390.21 (+1) y3 | 503.30 (+1) y4 | 600.35 (+1) y5 | |
| VGLPISQ*R | 438.76 (+2) | 397.21 (+1) y3 | 510.30 (+1) y4 | 607.35 (+1) y5 | ||
| Detection | ||||||
| TB18, Ricin | NDGTILNLYSGLVLDVR | 931.50 (+2) | 274.23 (+1) y2 | 858.52 (+1) y8 | 1021.63 (+1) y9 | |
| TB18, RCA | NDGTILNLYNGLVLDVR | 945.01 (+2) | 274.23 (+1) y2 | 885.52 (+1) y8 | 1048.58 (+1) y9 | |
| (TA24–SS–TB1), Ricin, RCA | CAPPPSSQF ADVCMDPEPIVR |
759.01 (+3) | 484.32 Tb1 (+1) y4 | 662.26 TA24 (+1) y6 | 710.42 Tb1 (+1) y6 | 1055.8 TA24 (+2) b8 |