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. Author manuscript; available in PMC: 2018 Mar 6.
Published in final edited form as: Neuroscience. 2017 Jan 9;344:380–393. doi: 10.1016/j.neuroscience.2016.12.055

Figure 5. Hair cell degeneration in Atoh1-CreER™; PjvkCKO and Prestin-CreERT2; PjvkCKO mice.

Figure 5

(A–I) Cochlear whole mounts of tamoxifen-treated Atoh1-CreER™Pjvkfl/+, Atoh1-CreER™Pjvkfl/fl and Prestin-CreERT2 Pjvkfl/fl mice at P60 were stained with phalloidin (F-actin, green), DAPI (nuclei, blue) and an antibody against myosin VIIa (red) to visualize hair cells. Apical, middle and basal cochlear turns for each genotype are shown. (J, K) Quantification of hair cell density in four different regions (apex, apex-mid, mid-base and base) along the cochlear sensory epithelium of tamoxifen-treated Atoh1-CreER™; PjvkCKO (J) and Prestin-CreERT2; PjvkCKO (K) mice at P60. Data are presented as mean ± SEM. *p < 0.05, *p < 0.01, ***p < 0.001 (Student’s t-test, n = 3 mice per group). Scale bars: (in I) A–I, 10 μm.