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. 2017 Feb 13;5:8. doi: 10.3389/fcell.2017.00008

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Copyright © 2017 Pastro, Smircich, Di Paolo, Becco, Duhagon, Sotelo-Silveira and Garat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative analysis of mRNA localization in epimastigote and metacyclic trypomastigotes of T. cruzi. Quantification of FISH assays of at least 30 parasites parasites using probes for: a receptor-type adenylate cyclase transcript, ID: TcCLB.511043.60, AdCyc, a surface protease GP63 transcript, ID: TcCLB.511211.90, GP63, the 60S ribosomal protein L44 transcript, ID: TcCLB.507105.40, L44, and polyA in epimastigotes (black) and metacyclic trypomastigotes (gray) (^*p < 0.0001).