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. 2017 Feb 13;5:8. doi: 10.3389/fcell.2017.00008

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Copyright © 2017 Pastro, Smircich, Di Paolo, Becco, Duhagon, Sotelo-Silveira and Garat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR of selected transcripts in nucleus and cytoplasmic fractions in T. cruzi epimastigotes. Transcript relative quantification was assessed by qRT-PCR in the four independent biological replicates. For normalization gapdh mRNA was used. Results are expressed as means of fold change between nuclear (N) and cytoplasmic (C) fractions. Standard errors are indicated. (GP-63, surface protease GP63, ID: TcCLB.506779.180; DGF, dispersed gene family protein 1, ID: TcCLB.509883.9; HSP60-Mit, chaperonin HSP60 mitochondrial, ID: TcCLB.507641.290; PK, protein kinase, ID: TcCLB.510343.15; Hypothetical, hypothetical protein, ID: TcCLB.506931.4; RBP, RNA-binding protein, ID: TcCLB.506649.80; RP-L44, ribosomal protein L24, ID: TcCLB.503611.20; PRPS, phosphoribosyl pyrophosphate synthetase, ID: TcCLB.508717.30). See Table S1 for specific primers used.