Figure 3. Direct interaction of the KIN peptide to CLV1 and TDR.

(a) Effects of 10 μM peptides on 10-day-old mutant stele width. Data represent mean values±s.d., with asterisks indicating a significant difference from mock treatment in each genetic background in two-tailed Welch's t-test (P<0.05, n=13–16, see Supplementary Data 1 for individual sample sizes). (b) Structure of the [¹²⁵I] ASA-KIN photo-affinity probe. (c) Photo-affinity labelling of TDR and CLV1 ectodomains with [¹²⁵I] ASA-KIN. Competition assays were performed with 1,000-fold excess amount of non-radio-labelled peptides as indicated above (comp.). Representative photos among two independent experiments are shown.