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. 2017 Feb 6;8:14118. doi: 10.1038/ncomms14118

Figure 2. BPIFA1 decreases airway smooth muscle (ASM) contractility by suppressing myosin light chain (MLC) phosphorylation.

Figure 2

Human ASMCs were grown in type I collagen matrices in 24-well plates. Gel contractions were measured as indicated over 60 min. (a) Human ASMCs were co-cultured with whole and denuded tracheal rings from Bpifa1+/+ or Bpifa1−/− mice for 48 h before contraction was induced with Ach. White arrows show the location of excised tracheas on the cultures. (c) ASMCs were pretreated with BPIFA1 (10 μM) or vehicle for 1 h and gel contraction was then measured±Ach (100 μM). Representative images of gel contraction are shown. (b,d) Summary of data from a and c, respectively, expressed as the % decrease in gel surface area at 60 min (all n=3 per group). (e) Representative immunoblots probed for total and phosphorylated MLC. (f) Mean densitometry taken from e (n=3). Data in b,d and f are mean±s.e.m. The data were analysed using one-way ANOVA followed by Tukey post hoc analysis. *P<0.05, **P<0.01.