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. 2017 Feb 6;8:14153. doi: 10.1038/ncomms14153

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(a) Examples of sapogenins from A. vulgaris including three heterocyclic cycloartanes. (b) Overlay of GC–MS total ion current chromatograms showing accumulation of triterpenoids produced in yeast strains expressing SlCAS together with CYP716A113 (red), SlCAS only (green), or CYP716A113 only (blue), compared with a control (empty) strain (black). (c) Overlay of GC–MS total ion current chromatograms showing accumulation of standard compounds and triterpenoids produced in yeast strains expressing single A. coerulea CYP716s in combination with GgBAS. Candidate-specific peaks are indicated with numbers (1) β-amyrin, (2) 16β-hydroxy β-amyrin, (3) erythrodiol and (4) oleanolic acid.