Skip to main content
. 2017 Jan 30;2017:1790808. doi: 10.1155/2017/1790808

Figure 5.

Figure 5

Low PP2A activity promoted the differentiation and proliferation in cardiac fibroblasts on FC. (a) The proliferation assay of fibroblasts with or without okadaic acid treatment on FC (p < 0.0002). (b) The proliferation assay of fibroblasts (p < 0.001). (c) Western blots showed the expressions of PP2Ac, PTEN, AKT, p-AKT, α-SMA, and actin at day 4 in shRNA-control or shRNA-β1 integrin transfected fibroblasts on FC. (d) The expressions of PTEN, α-SMA, actin, p-AKT, AKT, p-ERK, and ERK by Western blot in fibroblasts with or without okadaic acid treatment on FC. (e) Analysis of proliferation (left panel) and of the expressions of p-AKT, AKT, p-ERK, and ERK by Western blot in fibroblasts treated with control, UO126, wortmannin, or UO126 + wortmannin on FC at day 4 (right panel). (f) Analysis of PTEN activity in fibroblasts treated with or without okadaic acid treatment on FC. Representative immunoblots from three independent experiments are shown.