Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 6;8:14355. doi: 10.1038/ncomms14355

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Glial responses to axon injury in controls and highwire null (hiw^ΔN) animals after axotomy (1 day after bilateral antennal ablation, 1 dpant). Note glial membrane hypertrophy, activation of the of TRE reporter and upregulation of Draper (anti-Draper) in cohort controls (hiw^ΔN/+; TRE-RFP-16/+; repo-Gal4, UAS-mCD8::GFP/+). highwire null animals (hiw^ΔN/Y; TRE-RFP-16/+; repo-Gal4, UAS-mCD8::GFP/+), where axon degeneration is blocked, showed no detectable activation of glia. Dotted line delineates edge of the antennal lobe. Representative images were shown from five animals. Scale bar=10 μm. (b) Highwire was highly enriched in the synaptic region in the antennal lobe, but not in the surrounding glial cells (labelled with repo-Gal4, UAS-mCD8::GFP/+). Representative images were shown from three animals. (c) Glia require Draper to activate TRE reporter 1day after antennal ablation. +/+: TRE-eGFP-16; +/+. drpr^Δ5: TRE-eGFP-16; drpr^Δ5. Representative images were shown from three animals. Scale bar=10 μm. (d) Quantification of the increase of eGFP immunoreactivity in (c). n=15 each. Unpaired t-test, 2-tailed. ****: P<0.0001. (e) Ensheathing glial MARCM clones (green, outlined) responding to axotomy at day 1 post antennal ablation. Control ensheathing glial clones (+/+) became hypertrophic and exhibited strong TRE reporter activation (red) 1 day after antennal ablation, while draper null (drpr^Δ5) clones did not respond to axotomy (that is lack of membrane hypertrophy, and activation of TRE reporter) while neighbouring control cells were activated. +/+: TRE-RFP-16, UAS-mCD8::GFP/repo-FLP^6–2; FRT2A, FRT82B/tub-Gal80, FRT2A, repo-Gal4. drpr^Δ5: TRE-RFP-16, UAS-mCD8::GFP/repo-FLP^6–2; drpr^Δ5, FRT2A/tub-Gal80, FRT2A, repo-Gal4. Representative images were shown from four animals. Scale bar=10 μm. (f) TRE-RFP reporter activity in cortex glial MARCM clones (dotted line, green) 1 day after antennal ablation. Similar to ensheathing glia, cortex glial clones that lacked Draper (drpr^Δ5) failed to activate TRE reporter. Genotypes as in (e). Representative images were shown from four animals. Scale bar=10 μm.