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. 2017 Feb 6;8:14360. doi: 10.1038/ncomms14360

Figure 4. Disruption of FAK decreases survival signalling.

Figure 4

(a) Representative TUNEL of perigonadal WAT sections from 20- to 24-week-old mice fed HFD for 12–16 weeks (scale bar, 100 μm; arrows indicate TUNEL-positive nuclei) (n=4 mice). (b) Energy expenditure measured by oxygen consumption normalized to lean body mass (LBM) in mice fed HFD (n=3 control or 4 aP2FAK−/− mice). (c) Representative western blotting for cell survival signalling proteins in adipocytes from perigonadal WAT of HFD-fed mice with quantification (n=3 mice). (d) Representative PI staining of 3T3-L1 adipocytes day 7 after siRNA knockdown of FAK (scale bar, 100 μm; arrows indicate PI-positive nuclei) with quantification (n=3 replicates). (e) Relative macrophage F4/80 gene (Emr1) expression in whole perigonadal WAT of aP2FAK−/− versus control mice (n=6 mice). (f,g) Macrophage crown-like structures (arrow) seen with haematoxylin and eosin (H&E) staining with quantification (h) and fibrosis seen with Masson's trichrome staining (i) in perigonadal WAT sections from 24-week-old HFD-fed mice (scale bar, 100 μm). Data are mean±s.e.m. *P<0.05 by Student's t-test.