Figure 3. AR dimerizes in solution through the H5-H5' interface.

SPR analysis of AR-LBD self-association by kinetics (a) or affinity (b). The results of experiments conducted in duplicate are shown along with the respective calculated affinity constants. (c) Closeup of the core dimer interface highlighting the close proximity between the C687 Sγ atoms from both monomers. (d) BMOE-induced cross-linking of AR-LBD. The molecular masses (in kDa) of standard proteins are shown at the left side of the gel (MW). Notice detection of an AR-LBD dimer along with bands corresponding to higher-order aggregates in the presence but not in the absence of the crosslinker. (e,f) Representative MS/MS spectra identifying BMOE-crosslinked peptides that include residues C687 from both monomers. See also Supplementary Fig. 2 and Supplementary Table 1.