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. 2017 Jan 26;40(1):54–65. doi: 10.14348/molcells.2017.2258

Fig. 2. DDX53 regulates cancer stem cell-like properties of MDA-MB-231 cells.

Fig. 2

(A) MDA-MB-231 cells were transfected with the indicated siRNA (10 nM). At 48 h after transfection, Western blot analysis was carried out. (B) MDA-MB-231 (CD133+) cells were transfected with the indicated siRNA (10 nM) in the absence or presence of celastrol (2 μM) or cisplatin (150 μM), followed by tumor spheroid formation assays. * p < 0.05. (C) MDA-MB-231 (CD133−) cells were transfected with the indicated construct (1 μg). Western blot analysis was carried out. (D) MDA-MB-231 (CD133−) cells were transfected with the indicated construct (1 μg) in the absence or presence of celatsrol (2 μM) or cisplatin (150 μM), followed by tumor spheroid formation assays. *p < 0.05; **p < 0.005.