Figure 1. Representative video images of postconfluent HUVSMCs cultured in control smooth muscle cell medium or serum-free DMEM for another 2 and 4 days.

The images were taken under ×4 magnification. The mRNA level of smooth muscle а-actin (α-SMA) was measured by real-time PCR and quantified by 2^−ΔΔCt method for both control and serum-deprived cells.