Figure 5. In the presence of PSR replication licensing factors localize properly to the paternal chromatin during the first but not the second S-phase.

Top, PSR-carrying embryos during the first S-phase (left column) and metaphase of the first mitosis (right column) showing localization patterns of replication licensing factors, MCMs 2-7. Embryos were counterstained for H3K9me2,3 to show the initial appearance of this mark, which occurs after replication licensing. Bottom, wild type (left column) and PSR-carrying (right column) embryos stained for MCM2-7 and ORC2. While both factors localize properly to nuclei in wild type embryos and in maternally derived nuclei in PSR-carrying embryos, ORC2 was visibly absent from the PCM (red arrow) and MCMs were reduced on the PCM. Scale bar equals 12 μM. (p) and (m) mark the paternal and maternal nuclei, respectively.