Table 1. Primers used for RT-qPCR analysis and dsRNA synthesis.

Gene	Primer sequence (5′ to 3′)	Amplicon size (bp)	PCR efficiency (%)	R2
RT-qPCR
ylsAct	CATCCACGTAACCACCTACAA	224	105.4	0.999
	AGGCCAAGATAGATCCACCA
ylsEF1α	ACTACCCAGTGGTCCGAGTC	209	104.1	1.000
	AAGGTCTTGCCAGTGGTCTT
ylsGADPH	GCAAAGTCATTCCCGATCTT	196	103.6	0.996
	GAGACGATTTCTTCATCGCA
ylsRPS11	ACGGAATCCCATTCAAAGAG	167	102.5	0.999
	AGCTTGAGCAGCTACATTGG
ylsRPS15e	AAGCCAAGCCTAACGAGAAG	234	99	0.997
	GTGGAATGAAACGAGACGAA
ylsRPS15p	AGCTCTGAGGTCCAGATTGC	193	103.5	1.000
	TCAAGTGGGTCCATCTTTCA
ylsTub	TTGATTGCTCAGGTCGTCTC	196	115.7	0.995
	GGTCATCTCTTGGACGGAGT
ylsUBQ	CTTTGACGGGAAAGACGATT	156	92.8	0.998
	AATCGCTCAGAGTACGTCCA
NlylsSDH	AATGCCTGCTCGTTGATAAC	185	N.A.	N.A.
	ATGTCTTGCCCTAAGGTGCT
ylsArg4	CGAGTTCGAGCAGATTGAAA	70	N.A.	N.A.
	GATGGCAAATATGCCTGTTG
EdePRTase	CGAATCCAGACATGATCGAC	165	N.A.	N.A.
	AGCCGTCATCCAGTGATGTA
dsRNA synthesis
dsEdePRTase	T7-AAGAATCTACCGATCGCCCT	532	N.A.	N.A.
	T7-GGGATCTAATCAAGACGGCA
dsylsArg4-1	T7-GACATACGCGGCAGCATT	430	N.A.	N.A.
	T7-CGAGGCAGTAGGAAAGAAGC
dsylsArg4-2	T7-TCACCGAGACCCTACAATG	307	N.A.	N.A.
	T7-TCCGACACCGTCTTGACA
dsGFP	T7-CCTGAAGTTCATCTGCACCAC	355	N.A.	N.A.
	T7-TGATGCCGTTCTTCTGCTTGT

Note: T7 RNA polymerase promoter sequence (5′-TAATACGACTCACTATAGGG-3′). N.A., not applicable.