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. 2017 Feb 13;7:42114. doi: 10.1038/srep42114

Figure 2. Systemic deletion of CTGF reduced mRNA expression of fibrotic makers in anti-GBM nephritis.

Figure 2

(a) Serum creatinine and BUN levels. No significant difference between control and Rosa-CTGF cKO mice with nephritis. (b) Expression of Ctgf and Tgfb1 mRNA in the glomeruli of the kidney at 4 weeks after induction of anti-GBM nephritis. Glomerular Ctgf expression decreased by 80% in Rosa-CTGF cKO mice with nephritis compared with control mice with nephritis. Tgfb1 expression was also decreased in Rosa-CTGF cKO with nephritis. (c) Expression of Acta2, Fn1 and Itgav mRNA in the glomeruli. Gapdh mRNA expression was used as internal control. (d) Glomerular CTGF protein at 4 weeks after induction of anti-GBM nephritis by Western blotting (n = 4, each). β-actin was used as internal control. Full-length blots are presented in Supplementary Figure S10. Values are expressed as means ± s.e. *P < 0.05, **P < 0.01. Vehicle-treated control mice (n = 7), vehicle-treated Rosa-CTGF cKO mice (n = 7), control mice with anti-GBM nephritis (n = 7), Rosa-CTGF cKO mice with anti-GBM nephritis (n = 9). Veh, vehicle; GBM, anti-GBM nephritis.