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. 2017 Feb 13;7:42271. doi: 10.1038/srep42271

Figure 3. ALKBH3 and ALKBH5 have distinct roles in RNA demethylation.

Figure 3

The larger RNA fraction, smaller RNA fraction, tRNA, and mRNA were incubated in the absence (Mock) or presence of ALKBH3 or ALKBH5, enzymatically degraded to nucleosides, and then subjected to LC-ESI-MS/MS. The peak areas of methylated nucleosides were normalized to that of cytidine. (A) ALKBH5, but not ALKBH3, demethylated N6-meA in mRNA. (B) ALKBH3, but not ALKBH5, demethylated N6-meA in tRNA. (C) In the larger RNA fraction, ALKBH3 demethylated 3-meC and 1-meA. On the other hand, ALKBH5 demethylated only N6-meA. (D) In the smaller RNA fraction, ALKBH3 demethylated 3-meC, 1-meA, and N6-meA. On the other hand, ALKBH5 did not demethylate any of the methylated nucleosides. Data show means ± S.D. (n = 3). N.S.: p > 0.05, *p < 0.05, **p < 0.01.