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. 2017 Feb 13;7:42369. doi: 10.1038/srep42369

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(a) Maximum pulling force and (b) adhesion energy are increased in SCFS measurements upon acidification of NHE1-overexpressing cells (pH_e7.4: 0.22 nN (0.14/0.32 nN) and 0.97 fJ (0.5/1.36 fJ), N = 5, n = 62; pH_e7.0: 0.25 nN (0.17/0.35 nN) and 1.24 fJ (0.8/1.9 fJ), N = 5, n = 61; pH_e6.8: 0.27 nN (0.18/0.39 nN) and 1.25 fJ (0.8/2.07 fJ), N = 5, n = 67; pH_e6.4: 0.32 nN (0.24/0.5 nN) and 1.65 fJ (1.02/2.94 fJ), N = 5, n = 79). Force measurements were performed in paired experiments by exposing the same cells to different pH_e values. Statistical significance was tested using Kruskal-Wallis ANOVA and Mann-Whitney U test.