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. 2017 Feb 10;10(2):229–240. doi: 10.1016/j.tranon.2016.12.011

Figure 1.

Figure 1

Characterization of MSNs and VPA releasing profiling. (A) SEM image of MSNs. (B) TEM image of MSNs. (C) FTIR spectra of MSNs: nonfunctionalized (control), benzimidazole functionalized (BI-MSNs), amine functionalized (NH2/BI-MSNs), and folate grafted MSNs (FA/BI-MSNs). (D) Releasing profiling of beta-CD and VPA from FA/BI-MSNs in different condition. Release of VPA salt was monitored using HPLC-MS with positive mode. (E and F) Confocal microscopy images of HEK-293 (FR−, E) and C6 (FR+, F) cells after treatment with 10 μg/ml of MSN@FITC for 12 hours. Images are taken and merged under green channel from FITC (488/520 ± 10 nm) and blue channel from Hoechst 33258 (340/461 nm ± 10 nm). For HEK-293 cells where FR is not expressed, minimal unspecific binding of MSN@FITC could be found (E). For C6 cells expressing FRs, massive folic-MSN@FITC binding was observed (F).