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. 2017 Feb 10;10(2):229–240. doi: 10.1016/j.tranon.2016.12.011

Figure 4.

Figure 4

Flow cytometry of C6 and U87 cell apoptosis without irradiation. Flow cytometric analysis of cell apoptotic levels by PI and FITC–Annexin V fluorescence (A and B). The calculated apoptotic rates were shown in histograms (C and D). Without irradiation, no significant apoptosis was observed under VPA and MSNs treatment in both C6 and U87 cells. No distinguishable changes of apoptotic rate can be acquired from the calculation. There was also no significant apoptosis under VPA-MSNs treatment in pH 6.0 compared with VPA-MSNs treatment or the control in PBS (pH 7.4).