Figure 3.

Label-free profiling of ML349-biotin enriched proteins. (a) ML349-biotin enrichment relative to o-ML349-biotin. (b) ML349-biotin enrichment relative to azide-PEG₃-biotin. (c) o-ML349-biotin enrichment relative to azide-PEG₃-biotin reports few o-ML349-biotin enriched proteins. (d) ML349-biotin enrichment with or without (control) APT2 covalent inhibition by HDFP. Protein identifiers (Uniprot accession code) are shown for significantly enriched proteins quantified by at least three peptides. Dotted lines indicate a minimum 5-fold change and p-value less than 0.05. Enriched binding proteins from HEK-293T cell lysates are shown.