Table 1. Overview of some of the currently available tools/software dedicated to single-cell migration data.
| Software | Experimental setup, annotation | Free | Open Source | Cell tracking | Multiple conditions, technical replicates | Quality control | Statistics | Database | Multiscale analysis | Visualization of tracks | Multiparameter visualization | Homepage |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CellMissy single-cell module | + | + | + | − | + | + | + | + | SC, TC, CP | RP, HMP | RP, HG, AHG, BP, KDP, HMP | https://github.com/compomics/cellmissy |
| CellProfiler Tracer | − | + | + | + | + | + | − | + | TC, CP | RP, HMP | RP, HG, BP, HMP | http://cellprofiler.org/tracer/ |
| CellTracker | − | − | + | + | − | − | − | − | CP | RP | RP | http://celltracker.website/about-celltracker.html |
| Ibidi Chemotaxis and Migration Tool | − | + | + | + | − | − | + | − | CP | RP | RP, AHG | http://ibidi.com/xtproducts/en/Software-and-Image-Analysis/Manual-Image-Analysis/Chemotaxis-and-Migration-Tool |
| Icy PACT/Icy Track Manager | − | + | + | + | − | − | − | − | TC, CP | RP | RP, AHG | http://icy.bioimageanalysis.org/protocol/PACT:_a_Pipeline_for_Automated_Cell_Tracking |
| ImarisTrack | − | − | − | + | − | + | + | − | CP | RP | RP, HG | http://www.bitplane.com/imaris/imaristrack |
| MetaMorph | + | − | − | + | + | − | − | − | CP | RP | RP, HG | https://www.moleculardevices.com/systems/metamorph-research-imaging/metamorph-microscopy-automation-and-image-analysis-software |
| MotilityLab | − | + | − | − | − | − | + | − | CP | RP | BP | http://www.motilitylab.net |
| TrackMate | − | + | + | + | − | + | − | − | TC, CP | RP | RP, HG | https://imagej.net/TrackMate |
| Volocity | + | − | − | + | + | − | − | − | CP | RP | RP, HG | http://www.perkinelmer.com/lab-products-and-services/cellular-imaging/tracking-in-volocity.html |
Multiscale analysis SC: step-centric TC: trajectory-centric CP: cell population Visualization of tracks/Multiparameter visualization RP: rose plot HMP: heat-map plate AHG: angular histogram BP: boxplot HG: histogram KDP: kernel density plot.