Table 2. Description of the experimental data used in this article to demonstrate the functionalities of the single-cell analysis module in CellMissy.

Experiment number	1	2
Cell line	Ba/F3	MDA-MB-231
Cell type	B-cell	Mammary adenocarcinoma
#conditions tested	6	10
Conditions	Bcr-Abl p190a, Bcr-Abl p210, or Bcr-Abl p210mut; ± y27632	Control, EGF, crizonibc, foretinibc, gefitinibd, lapatinibd
#replicates/condition	2	6
Assay type	Single cell migration of cells embedded in Matrigel (2 mg/ml), cells enriched in one focal plane	Cell zone exclusion assaye in 3D collagen matrix (2 mg/ml)
Time-lapse: imaging type, duration, interval, magnification	Phase-contrast xyt imaging, 6 h, 1.5 min, 20x	Phase-contrast xyt imaging, 48 h, 20 min, 10x
Image processing: software/output used	CELLMIAb, (x, y) trajectories	CELLMIAb, (x, y) trajectories of cells escaping form the confluent sheet

^aBcr-Abl oncogene variants (see ref. 27); y27632: Rock inhibitor used at 10 μM.

^bIn house development tool (collaboration Dci-Labs Belgium).

^cc-Met inhibitors (different concentrations tested).

^dEpidermal growth factor (EGF)-inhibitors (different concentrations tested).

^esee ref. 29.