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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Biomaterials. 2016 Feb 8;87:82–92. doi: 10.1016/j.biomaterials.2016.02.008

Fig. 2.

Fig. 2

CD47 Quantification. (A) The modification chemistry summarized in Figure 1 was utilized to facilitate quantification. Following PEI-PDT addition, primary amines were acetylated using sulfo-NHS-Acetate. recCD47 and pepCD47 were biotinylated using Sulfo-NHS-LC-LC-Biotin. Biotinylated recCD47 and pepCD47 were then reacted with the steel surfaces. ABC reagent and Ultra TMB substrate were used to determine the concentration of CD47 by comparison to a standard curve (inset) prepared using escalating amount of biotinylated IgG assayed in the same manner. (B) CD47 concentration on steel surfaces in the presence or absence of bi-functional cross-linking agent (sulfo-LC-SPDP).