Figure 6.
The uuat1-2 Mutant Line Displays an Early Stem Elongation Phenotype and an Increase in Methylesterification but Shows No Changes in Sugar Content in Stem Cell Walls.
(A) The uuat1-2 mutant displays early stem elongation when compared with the wild-type Col-0. Seven-week-old wild-type Col-0 and uuat1-2 plants show a pronounced difference in stem height. This difference disappears once the plants reach their adult state. This phenotype was observed in three biological replicates.
(B) Determination of the degree of methylesterification in stems. Error bars represent se (n = 8) from two biological replicates. Asterisks represent significant difference from wild-type Col-0 using ANOVA and Tukey tests (P < 0.05).
(C) High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used to quantify the cell wall extract monosaccharide composition from wild-type Col-0 and uuat1-2 stems (20–24 cm). Error bars represent se (n = 6) from three biological replicates.
(D) Ratio of GlcA/Xyl content of xylan products digested with GH11 xylanase. AIR material from basal stems of wild-type Col-0 and uuat1-2 was used to determine the frequency of GlcA branches on the xylan backbone using PACE. The quantity of each of the oligosaccharides released by GH11 xylanase [Xyl, (Xyl)2, GlcA-(Xyl)4/MeGlcA(Xyl)4] was calculated and the GlcA/Xyl ratio determined. Error bars represent se (n = 9) from three biological replicates.