Table 1. Sugar Composition of Seeds plus Adherent Mucilage (Seed+AM) and Extracted SM from Wild-Type Col-0 and uuat1-2 Plants.
| Structure | Sugar | Wild-Type Col-0 | uuat1-2 |
|---|---|---|---|
| Seed+AM | GalA | 20.67 (0.21)* | 19.68 (0.42)* |
| Rha | 21.77 (0.77)* | 20.02 (0.45)* | |
| Fuc | 1.74 (0.05) | 1.72 (0.03) | |
| Ara | 41.21 (1.59) | 39.62 (1.06) | |
| Xyl | 11.37 (0.56) | 11.45 (0.47) | |
| Man | 3.44 (0.12) | 3.69 (0.16) | |
| Gal | 30.23 (0.40) | 29.06 (1.13) | |
| Glc | 7.43 (0.26) | 8.18 (0.29) | |
| GlcA | 2.44 (0.08) | 2.55 (0.07) | |
| Total Seed+AM | 140.30 (2.80) | 135.96 (1.92) | |
| SM | GalA | 5.66 (0.21)* | 4.56 (0.22)* |
| Rha | 8.65 (0.49)* | 6.76 (0.43) * | |
| Ara | 0.08 (0.02) | 0.09 (0.01) | |
| Xyl | 0.43 (0.02)* | 0.34 (0.02)* | |
| Gal | 0.31 (0.04) | 0.26 (0.02) | |
| Total SM | 15.15 (0.64) | 11.99 (0.95) |
To analyze monosaccharide composition, a water-soluble extraction was used to isolate the SM fraction. The adherent mucilage cannot be detached from the seed and form the seed + soluble mucilage fraction (seed+AM). Sugar content was obtained using HPAEC-PAD from seed+AM and from SM. Values are in mg/g of dry seeds and are the means of three biological replicates. Standard errors are shown in parentheses for two technical replicates each. Asterisks indicate significant statistical differences using the Wilcoxon test (P < 0.05).