Figure 6.

ES16 Inhibits Root Elongation and Gravitropic Response without Affecting Lateral Root Organogenesis.

(A) Growth phenotype of 5-d-old Col seedlings on different dosages of ES16 or DMSO.

(B) Root growth phenotype after 90° reorientation for 12 h on 0.4% DMSO- or 40 µM ES16-supplemented medium.

(C) Auxin redistribution as labeled by DR5:N7-VENUS in roots in response to gravity stimulus on 0.4% DMSO or 40 µM ES16 medium after reorientation for 90 min.

(D) Lateral root initiation visualized by DR5:N7-VENUS on medium supplemented with 10 µM NAA together with either 0.4% DMSO or 40 µM ES16.

(E) Quantification of root length on medium with different dosages of ES16 as shown in (A). Day 0 was scored when seeds started to germinate, and growth phenotypes were recorded every day up to 7 d; 80 seedlings were scored for each concentration.

(F) Time course of root curvature after gravity stimulation in the presence of either 0.4% DMSO or 40 µM ES16. Angles of curvature were measured every 2 h up to 20 h after reorientation.

(G) Quantification of lateral root number after 9 d on either DMSO medium or different dosages of ES16 medium. Lateral roots longer than 0.5 mm were chosen for monitoring; 80 seedlings were used for quantification for each concentration. Bars represent mean values with sd.

Bars =1 cm in (A), 20 µm in (C), and 50 µm in (D).