RSL4 Induces Root Hair Growth in the Non-Root-Hair Cell Position and in the rhd6 Mutant.
(A) Root images of control (Cont; ProE7:YFP) and non-root-hair cells expressing transformants of RSL4 (ProGL2:RSL4, RSL4) in the wild-type (WT) background. Bars = 100 μm.
(B) Quantitative analysis for RSL4-induced root hair formation in the non-root-hair cell position. Relative number of root hair cells and non-root-hair cells of total counted epidermal cells in the root hair cell position (H) or in the non-root-hair cell position (N) was estimated. Error bars indicate ±se from 970 to 980 epidermal cells (from 20–21 roots for the transformants).
(C) Root images of the rhd6-3 mutant and ProPIN2:RSL4 in the rhd6-3 mutant background. The values are relative to the control value and are significantly different (***P < 0.0005; Student’s t test). Bars = 200 μm.
(D) Quantitative analysis of root hair formation in the root hair cell position as described in (B). Error bars indicate ±se from 600 to 1470 epidermal cells (from 12–26 roots for the transformants). Statistically significant differences are denoted with different letters (one-way ANOVA with Tukey’s unequal N HSD post hoc test, P < 0.05).