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Enzymatic parameters for Fluc-catalyzed light emission.
| Compound | Apparent Km (µM) | Relative kcata |
|---|---|---|
| D–Luc | 3.07 ± 0.80 | 100 ± 0.3 |
| 4'–BrLuc | 2.47 ± 0.92 | 1.8 ± 0.4 |
| 5'–BrLuc | 6.72 ± 0.31 | 104 ± 2.2 |
| 7'–BrLuc | 17.3 ± 8.8 | 1.9 ± 0.4 |
Expressed as percent relative to D–Luc
