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. 2017 Feb 13;8:168. doi: 10.3389/fmicb.2017.00168

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Copyright © 2017 Wang, Zhang, Wang, Gadahi, Xu, Yan, Song and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot for the recombinant TgEF-1α and native protein of TgEF-1α. Lane M: standard protein molecular weight marker; (A) lane 1: Rrecombinant protein TgEF-1α probed by serum from chickens experimentally infected with Toxoplasma gondii as primary antibody; lane 2: Rrecombinant protein TgEF-1α probed by serum of normal chickens as primary antibody. (B) Lane 1: Sonatic extract of T. gondii tachyzoites probed by rat anti-rEF-1α antisera as primary antibody; lane 2: Sonatic extract of T. gondii tachyzoites probed by serum of normal rat as primary antibody.