Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Coomassie assay calibration curves to determine the protein concentration CP of solutions with (a) high concentration and (b) low concentrations by measuring the offset-corrected absorption A at 595 nm. The fitting curves are (a) A(CP) = 0.029 + (0.001 mL/μg)·CP − (3 × 10−7 mL2/μg2)·CP2, and (b) A(CP) = −0.005 + (0.017 mL/μg)·CP – (6.789 × 10−4 mL2/μg2)·CP2 – (1.97 × 10−5 mL3/μg3)·CP3 and the coefficients of determination (r2) are equal to 0.994 and 0.989, respectively.
