Figure 5.

Calibration curve in which the fluorescence intensity I at 519 nm is determined for protein solutions of different concentration C_P. Data are shown for antibodies against (a) horseradish peroxidase (HRP) and (b) vascular endothelial growth factor (VEGF). A linear fit leads to the following correlation between fluorescence intensity I and concentration C_P: (a) I(C_P) = I₀ + (ΔI/ΔC_P)·C_P = −464.28 + (4447.1 mL/µg)·C_P; (b) I(C_P) = −3549.4 + (5498.3 mL/µg)·C_P. The coefficients of determination (r²) are equal to 0.998 and 0.995 for HRP and VEGF, respectively.