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. 2014 Sep 17;4(3):827–843. doi: 10.3390/nano4030827

Figure 6.

Figure 6

(a) Sequence alignment illustrating the construction of the chimeric hydrophobin NChi2 from the proteins NC2 and EAS (named for its easily wettable spore phenotype). The Cys7–Cys8 region of NC2 is shorter than the corresponding region in EAS and EAS∆15, this difference is indicated with a dotted line. The 15-residue deletion from EAS, to generate EAS∆15, is indicated by a solid line. The conservation of the amyloidogenic region between EAS, EAS∆15 and NChi2 is indicated in red; (b) TEM image of negatively stained NChi2 rodlets formed under conditions of low pH (=2.5) and elevated temperature (45 °C) with extended shaking. (c) AFM image of NChi2 dried down from pH = 2.5 at 45 °C and then washed with MQW for 5 min.