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. 2017 Feb 4;23:640–648. doi: 10.12659/MSM.898740

Figure 2.

Figure 2

miR-495 inhibited cell migration and invasion in BGC-823 and HGC-27 cell lines and regulated EMT factors. Pre-miR-495, cells were transfected with miR-495 precursor to overexpress miR-495. miR-495 ASO, cells transfected with miR-495 antisense oligo (ASO) to inhibit miR-495. Pre-ASO control, the corresponding control group of pre-miR-495 or miR-495 ASO. qRT-PCR, Western blot and Transwell assays were performed at 48 hours after transfection. (A) miR-495 was successfully promoted or inhibited by cell transfection in BGC-823 and HGC-27 cells. (B) miR-495 inhibited, and its ASO promoted, cell migration in BGC-823 and HGC-27 cells. (C) miR-495 inhibited, and its ASO promoted, cell invasion in BGC-823 and HGC-27 cells. (D) miR-495 upregulated CDH1 and downregulated VIM and ACTA2 mRNA levels in BGC-823 cells. (E) miR-495 upregulated CDH1 and downregulated VIM and ACTA2 protein levels in BGC-823 cells. GAPDH mRNA and protein were used as internal references for qRT-PCR and Western blot, respectively. * p<0.05. ** p<0.01. *** p<0.001. CDH1 – E-cadherin. VIM – vimentin. ACTA2 – alpha smooth muscle actin.