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. 2017 Feb 4;23:640–648. doi: 10.12659/MSM.898740

Figure 4.

Figure 4

HMGA2 is upregulated in gastric cancer tissue and promotes cell migration and invasion in gastric cancer cell lines. (A) qRT-PCR showing relative HMGA2 mRNA level in gastric cancer tissue and the adjacent normal tissue. (B) Western blot showing HMGA2 protein level in gastric cancer tissue and the adjacent normal tissue. (C) HMGA2 protein level was successfully elevated by HMGA2 overexpression vector (HMGA2) and inhibited by its specific siRNA (si-HMGA2). (D) Transwell assay indicating that HMGA2 increased migrated cells in BGC-823 and HGC-27. (E) Transwell assay indicating that HMGA2 increased invasive cells in BGC-823 and HGC-27. (F) HMGA2 promoted CDH1 and inhibited VIM and ACTA2 mRNA levels in BGC-823 cells. (G) HMGA2 promoted CDH1 and inhibited VIM and ACTA2 protein levels in BGC-823 cells. GAPDH mRNA and protein were used as internal references for qRT-PCR and Western blot, respectively. * p<0.05. ** p<0.01. *** p<0.001. HMGA2, high mobility group AT-hook 2. CDH1 – E-cadherin. VIM – vimentin. ACTA2 – alpha smooth muscle actin.