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. 2017 Feb 7;12:1047–1064. doi: 10.2147/IJN.S115215

Figure 4.

Figure 4

The cellular uptake of HD micelles/DOX micelles in MCF-7/Adr cells.

Notes: (A) Flow cytometry curves and graphs of fluorescence intensity of intracellular DOX after incubation with HD micelles/DOX micelles with different formulations for 6 h at 37°C. The weight of PHIS-PEG2000 in the total weight of the two polymers: 50%, 65% and 80%. (B) Confocal microscopy images of intracellular DOX after incubation with HD micelles/DOX (the weight of PHIS-PEG2000 in the total weight is 65%) for 6 h at 37°C. Red represents fluorescence of DOX. (C) Flow cytometry curves and graphs of fluorescence intensity of intracellular DOX after incubation with HD micelles/DOX micelles for 2, 4 and 6 h at 37°C. The concentration of DOX was 1 μg/mL. (D) Flow cytometry curves and graphs of fluorescence intensity of intracellular DOX after incubation with HD micelles/DOX micelles at different DOX concentrations of 1, 2 and 10 μg/mL for 3 h at 37°C.

Abbreviations: DOX, doxorubicin; PHIS-PEG2000, poly(l-histidine)-coupled polyethylene glycol-2000; h, hour; HD micelles, PHIS-PEG2000 and DSPE-PEG2000 hybrid micelles; DSPE-PEG2000, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-2000.