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. 2017 Feb 7;12:1047–1064. doi: 10.2147/IJN.S115215

Figure 5.

Figure 5

The expression of TfRs and cellular uptake of 7-pep HD micelles/DOX in MCF-7/Adr cells.

Notes: (A) Expression of TfRs in MCF-7/Adr cells by immunofluorescence. Green represents TfRs; blue represents nuclei of MCF-7/Adr cells. The negative controls were conducted by incubation with 1% BSA instead of anti-TfR antibody to rule out the possibility of nonspecific binding. (B) Flow cytometry curves and graphs of fluorescence intensity of intracellular DOX after incubation with DOX-loaded HD micelles and 7pep-modified HD micelles with different modification rates (10%, 30%, 50% and 60%) for 6 h at 37°C. (C) Confocal microscopy images of intracellular DOX after incubation with DOX-loaded HD micelles and 7pep-modified HD micelles with 60% modification rate for 6 h at 37°C. Red represents fluorescence of DOX.

Abbreviations: TfR, transferrin receptor; BSA, bovine serum albumin; DOX, doxorubicin; h, hour; HD micelles, PHIS-PEG2000 and DSPE-PEG2000 hybrid micelles; DSPE-PEG2000, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-2000; PHIS-PEG2000, poly(l-histidine)-coupled polyethylene glycol-2000; 7-pep HD micelles, PHIS-PEG2000 and 7pep-DSPE-PEG2000 hybrid micelles; 7pep, HAIYPRH, a TfR ligand.