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. 1991 Dec 1;88(23):10951–10955. doi: 10.1073/pnas.88.23.10951

T cells expressing the V beta 1 T-cell receptor are required for IgA production in the chicken.

J Cihak 1, G Hoffmann-Fezer 1, H W Ziegler-Heibrock 1, H Stein 1, B Kaspers 1, C H Chen 1, M D Cooper 1, U Lösch 1
PMCID: PMC53050  PMID: 1835793

Abstract

While alpha beta T cells in mammals may express one of many variable (V) gene families in the beta locus, chickens have only two V beta gene families. The avian V beta 2+ T cells are recognized by the T-cell receptor 3 (TCR3) monoclonal antibody and V beta 1+ T cells are recognized by the TCR2 antibody, which we used to selectively suppress development of V beta 1+ T cells in order to examine their functional role. Suppression was accomplished by multiple injections of anti-TCR2 antibodies beginning in embryonic life and perpetuated by thymectomy 8 days after hatching. Young birds thus depleted of V beta 1+ T cells had greater than normal numbers of V beta 2+ T cells and appeared as healthy as thymectomized and untreated controls. While production of IgM and IgG antibodies was unimpaired, IgA antibody production was severely compromised in the V beta 1-depleted birds. The levels of secretory IgA in bile and lung lavage fluid were reduced 1000- to 10,000-fold and secretory IgA antibodies were not produced in response to mucosal immunization. B-cell production of IgA antibodies thus appears to require T cells expressing the V beta 1 genes, whereas T cells that express the V beta 2 genes lack this capacity.

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Selected References

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