Table 1.
Identification and quantification of MS peaks in EV from PC9 or PC9R cells in positive ion mode.
| m/z | pa) | Ratiob) | Assignment |
|---|---|---|---|
| Overexpressed in extracellular vesicles of PC9R cell | |||
| 754.5 | 2.00 × 10−1 | 1.03 | PC {34:4} [M+H]+ |
| 720.6 | 5.23 × 10−3 | 1.05 | PC{O-32:0}[M+H]+ |
| 706.6 | 1.72 × 10−13 | 1.2 | PC {30:0} [M+H]+ |
| 756.5 | 1.64 × 10−10 | 1.22 | PC {34:3} [M+H]+ |
| 742.5 | 1.24 × 10−15 | 1.36 | PC {O-34:3} [M+H]+/PC {P-34:2} [M+H]+ |
| 750.6 | 3.40 × 10−21 | 1.41 | PC {34:6} [M+H]+ |
| 834.7 | 4.97 × 10−20 | 1.45 | PC {40:6} [M+H]+ |
| 692.6 | 2.14 × 10−31 | 1.56 | PC{O-30:0}[M+H]+ |
| 804.6 | 2.05 × 10−19 | 1.61 | PC {38:7} [M+H]+ |
| 722.5 | 1.50 × 10−32 | 1.69 | PC {30:3} [M+Na]+ |
| 550.5 | 1.84 × 10−20 | 2.39 | LPC {20:1} [M+H]+ |
| 806.7 | 1.16 × 10−37 | 2.4 | PC {36:3} [M+Na]+/PC {38:6} [M+H]+ |
| 770.6 | 6.76 × 10−49 | 6.06 | PC {O-36:3} [M+H]+/PC {P-36:2} [M+H]+ |
| 912.7 | 7.03 × 10−35 | 6.96 | PC {44:6} [M+Na]+ |
| 728.5 | 1.28 × 10−44 | 9.25 | PC {32:3} [M+H]+ |
| 832.7 | 5.16 × 10−47 | 11.76 | PC {40:7} [M+H]+ |
| 884.7 | 6.0448 × 10−47 | 13.75 | PC {42:6} [M+Na]+ |
| Underexpressed in extracellular vesicles of PC9R cell | |||
| 732.6 | 9.18 × 10−1 | 0.99 | PC {32:1} [M+H]+ |
| 746.5 | 6.04 × 10−1 | 0.99 | PC {O-34:1} [M+H]+/PC {P-34:0} [M+H]+ |
| 782.6 | 2.58 × 10−1 | 0.97 | PC {34:1} [M+Na]+/PC {36:4} [M+H]+ |
| 718.7 | 3.15 × 10−3 | 0.93 | PC {O-32:1} [M+H]+/PC {P-32:0} [M+H]+ |
| 704.6 | 4.04 × 10−4 | 0.93 | PC {30:1} [M+H]+ |
| 820.6 | 9.04 × 10−3 | 0.91 | PC {40:5} [M+H]+ |
| 768.6 | 4.89 × 10−9 | 0.88 | PC {O-36:4} [M+H]+/PC {P-36:3} [M+H]+ |
| 780.6 | 3.33 × 10−7 | 0.88 | PC {36:5} [M+H]+ |
| 760.6 | 1.08 × 10−6 | 0.85 | PC {34:1} [M+H]+ |
| 787.8 | 9.40 × 10−18 | 0.82 | SM{d40:1}[M+H]+ |
| 703.6 | 1.1716 × 10−18 | 0.75 | SM {d34:1} [M+H]+ |
| 725.5 | 1.70 × 10−19 | 0.75 | SM {d34:1} [M+Na]+ |
| 522.3 | 8.13 × 10−9 | 0.74 | LPC {18:1} [M+H]+ |
| 810.6 | 2.10 × 10−25 | 0.73 | PC {36:1} [M+Na]+/PC {38:4} [M+H]+ |
| 675.6 | 1.42 × 10−26 | 0.73 | SM {d32:1} [M+H]+ |
| 774.6 | 1.12 × 10−27 | 0.71 | PC {34:5} [M+Na]+ |
| 812.6 | 1.14 × 10−22 | 0.71 | PC {38:3} [M+H]+ |
| 798.6 | 1.56 × 10−19 | 0.71 | PC {34:1} [M+K]+ |
| 759.7 | 1.41 × 10−23 | 0.7 | PC {38:1} [M+H]+ |
| 794.6 | 1.42 × 10−18 | 0.69 | PC {O-38:5} [M+H]+/PC {P-38:4} [M+H]+ |
| 518.3 | 6.30 × 10−15 | 0.68 | LPC {18:3} [M+H]+ |
| 504.5 | 1.12 × 10−16 | 0.62 | LPC {14:1} [M+K]+ |
| 649.5 | 2.01 × 10−9 | 0.55 | SM {d30:0} [M+H]+ |
| 546.4 | 1.16 × 10−24 | 0.55 | LPC {20:3} [M+H]+ |
| 562.4 | 5.08 × 10−23 | 0.54 | LPC {18:0} [M+K]+ |
| 796.6 | 1.85 × 10−30 | 0.53 | PC {O-38:4} [M+H]+/PC {P-38:3} [M+H]+ |
| 572.6 | 5.08 × 10−21 | 0.51 | LPC {22:4} [M+H]+ |
| 544.3 | 1.07 × 10−49 | 0.28 | LPC {20:4} [M+H]+ |
| 758.6 | 3.07 × 10−47 | 0.19 | PC {34:2} [M+H]+ |
| 808.6 | 4.13 × 10−54 | 0.15 | PC {38:5} [M+H]+ |
| 785.7 | 3.58 × 10−59 | 0.08 | SM{d40:2}[M+H]+ |
Peak position was indicated by the m/z ratio. MS peaks were assigned using lipid databases, Lipid Search or LIPID Metabolites and Pathways Strategy (LIPID MAPS) database (www.lipidmaps.org).
a)
p-value <0.01, fold change>1.5.
b)
Ratio, extracellular vesicles of PC9R cell/PC9 cell ratio.
P-, plasmenyl; O-, plasmanyl.
PC, phosphatidylcholines.
P-LPC, lysophosphatidylcholines.
P-SM, sphingomyelines.