Figure 1.

Schematic representation of the miR-145 locus. (a) Both premiR143 and premiR145 (grey boxes) are transcribed from a large primary transcript (miR143HG; white box) from chromosome 5q32, in between the genes encoding interleukin 17 beta and casein kinase 1A1. (b) Eleven potential methylation sites surrounding the premiR145 start site. Specific CpG sites were analysed using multiple pyrosequencing assays, sequences of which can be found in Table 1. (c) Six potential methylation sites flanking the miR143HG coding sequence, with pyrosequencing assays indicated and described in Table 1.