Table 1.
Primer sequences used in pyrosequencing assays.
| Region | CpG sites | Primer sequences |
|---|---|---|
| miR-145, assay 1 | −189, −175, −148, −112, −109, −106 | Forward: GAGATTGGGGAATATATATGAGT |
| Reverse: [Biotin]-TCTTCTACATCCAACCCCATCTAT | ||
| Sequencing: GAGGGTAGTTTTGGG | ||
| miR-145, assay 2 | −189, −175, −148, −112, −109, −106 | Forward: [Biotin]-GGAGATTGGGGAATATATATGAGT |
| Reverse: AAACCAACTAAAATTCTCTTCTACAT | ||
| Sequencing: ATTCTCTTCTACATCCAAC | ||
| miR-145, assay 3 | −148, −112, −109, −106 | Forward: GGTATTTTTTAGGGTAATTGAAGTTT |
| Reverse: [Biotin]-TCTACATCCAACCCCATCTATAACAA | ||
| Sequencing: ATTATTTTTTTTTAGAGTAATAAGT | ||
| miR-145, assay 4 | −32, −29, −20, −6, +13 | Forward: GGGTTGGATGTAGAAGAGAATTTTA |
| Reverse: [Biotin]-TCCAAAAATCCCCATCTTAACAT | ||
| Sequencing: ATTTTAGTTGGTTTTTAGGGATA | ||
| miR143HG, assay 5 | −106, 79, −31, −26, −16 | Forward: TTTTTTTTTAGGGGGTAAAAGTAATATT |
| Reverse: [Biotin]-CCTAACCACAAAAACCACTCTAC | ||
| Sequencing: AGGGGGTAAAAGTAATATTT | ||
| miR143HG, assay 6 | −31, −26, −16, +46 | Forward: GGGGGTGTTTGGGTTAAT |
| Reverse: [Biotin]-TAAACCCCCCTCCCCTTAAT | ||
| Sequencing: TTTGGGAGGGTTTAG | ||
| miR143HG, assay 7 | −106, 79, −31, −26, −16 | Forward: GGTTTTTGGTGGTTGGAGTTAAG |
| Reverse: [Biotin]-ACCACAAAAACCACTCTACC | ||
| Sequencing: AGGGGGTAAAAGTAATATT |